Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Stroke Volume01:27

Regulation of Stroke Volume

5.2K
The regulation of stroke volume, which is the amount of blood the heart pumps out during each heartbeat, is critical for maintaining a healthy circulatory system. Stroke volume is influenced by three main factors: preload, contractility, and afterload.
Preload refers to the degree of stretch on the heart before it contracts. It's analogous to the stretching of a rubber band; the more it's stretched, the more forcefully it snaps back. This concept is encapsulated in the Frank-Starling law of the...
5.2K
Cardiac Output and Stroke Volume01:11

Cardiac Output and Stroke Volume

4.9K
Cardiac output (CO) is an integral aspect of human physiology, reflecting the heart's efficiency and responsiveness to the body's needs. It represents the volume of blood that the left or right ventricle ejects into the aorta or pulmonary trunk each minute. The CO is calculated by multiplying the heart rate (HR)—the number of heartbeats per minute—by the stroke volume (SV)—the amount of blood pumped out with each heartbeat.
In an average resting adult male, the typical cardiac...
4.9K
Methods of Classification and Identification01:28

Methods of Classification and Identification

1.2K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.2K
Cardiac Output II: Effect of Stroke Volume on Cardiac Output01:22

Cardiac Output II: Effect of Stroke Volume on Cardiac Output

3.4K
Cardiac output (CO), the amount of blood the heart pumps per minute, is a parameter in cardiovascular physiology determined by stroke volume and heart rate. Stroke volume, the amount of blood pushed from one of the ventricles per heartbeat, is influenced by preload, afterload, and contractility.
Preload
Preload refers to the initial elongation of the cardiac myocytes before contraction and is related to the volume of blood filling the heart at the end of diastole, or end-diastolic volume. The...
3.4K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

8.9K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.9K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A 3D Human Bone and Bone Marrow-on-a-Chip Model for In Vitro Bone Remodeling and Immune Cell Maintenance.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

TGF-β and IL-2 differentially shape T follicular regulatory cell differentiation and stability in vitro.

Cellular & molecular immunology·2026
Same author

Hierarchical composite outcomes in acute ischaemic stroke with large infarct: a win ratio analysis of the TENSION trial.

European stroke journal·2026
Same author

The World Brain Health Forum: breaking silos for co-ordinated action.

Nature reviews. Neurology·2026
Same author

Circulating sphingosine-1-phosphate depletion is associated with endothelial activation and altered brain-endothelial S1P pathway expression in ischemic stroke.

Fluids and barriers of the CNS·2026
Same author

Large Core Stroke Thrombectomy Is Safe and Effective Regardless of Prior Antithrombotic or Thrombolytic Treatment: A Secondary Analysis of the Randomized TENSION Trial.

Journal of the American Heart Association·2026
Same journal

A Super-Resolution Approach for Astrocyte-Specific Molecular Imaging Reveals the Nanoscale Distribution of Monoacylglycerol Lipase, the Metabolic Node Between Endocannabinoid and Prostaglandin Signaling.

Glia·2026
Same journal

Blocking CK2α-Bclaf1 Preserves Oligodendrocytes After Neonatal Hypoxic Injury.

Glia·2026
Same journal

Molecular Characteristics and Differentiation Control Mechanisms of Bergmann Glia-Like Progenitors in the Postnatal Mouse Cerebellum.

Glia·2026
Same journal

Alzheimer's Protection by PLCγ2 Compacts Plaques, Redistributes Microglia, and Protects Synapses in App<sup>NL</sup> <sup>-G-F</sup> Mice.

Glia·2026
Same journal

Single-Nucleus Multiomic Analysis Reveals Immune-Metabolic Reprogramming Consistent With Maladaptive Trained Immunity in HIV-Associated Neurocognitive Disorders.

Glia·2026
Same journal

Plasma Membrane Remodeling During Microglial Activation: A Hypothesis Linking Microglial Shape and Lipid Droplet Formation.

Glia·2026
See all related articles

Related Experiment Video

Updated: Jan 31, 2026

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
15:04

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity

Published on: May 5, 2009

25.8K

Stroke target identification guided by astrocyte transcriptome analysis.

Cordula Rakers1, Melvin Schleif1, Nelli Blank1

  • 1German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany.

Glia
|December 27, 2018
PubMed
Summary
This summary is machine-generated.

Reactive astrocytes in stroke can be both protective and harmful. This study identified key genes, including Stat3, that influence astrocyte behavior, revealing potential therapeutic targets for stroke treatment.

Keywords:
astrogliaischemianext generation sequencing

More Related Videos

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

30.6K
Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents
09:42

Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents

Published on: August 7, 2013

10.8K

Related Experiment Videos

Last Updated: Jan 31, 2026

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
15:04

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity

Published on: May 5, 2009

25.8K
Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

30.6K
Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents
09:42

Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents

Published on: August 7, 2013

10.8K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Pathology

Background:

  • Astrocytes are crucial for brain function but can become detrimental in conditions like stroke.
  • The molecular mechanisms behind reactive astrogliosis remain unclear.

Purpose of the Study:

  • To investigate the molecular changes in astrocytes during stroke.
  • To identify astrocyte-specific therapeutic targets for stroke.

Main Methods:

  • Used the RiboTag technique to isolate astrocyte-specific mRNAs after transient middle cerebral artery occlusion (tMCAO) in mice.
  • Generated a stroke-specific astroglial translatome database.
  • Analyzed differentially expressed genes and transcription factors, focusing on Stat3.

Main Results:

  • Identified 1,003 differentially expressed genes and 38 transcription factors in reactive astrocytes.
  • Found enrichment of neuroprotective A2 phenotype transcripts alongside potentially neurotoxic genes.
  • Demonstrated that astrocyte-specific deletion of Stat3 reduced stroke volume and improved motor function.

Conclusions:

  • Reactive astrocytes exhibit a complex molecular profile with both beneficial and detrimental components.
  • Stat3 plays a critical role in stroke pathogenesis mediated by astrocytes.
  • Targeting astrocyte-specific pathways, like Stat3, offers a promising therapeutic strategy for stroke.