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Ethanol influence on gingival fibroblasts - a real-time in vitro study
Marzena Wyganowska-Świątkowska1, Agnieszka Nowak2, Elżbieta Paszyńska3
1Department of Conservative Dentistry and Periodontology, University of Medical Sciences, Poznan, Poland. marzena.wyganowska@periona.pl.
Annals of Agricultural and Environmental Medicine : AAEM
|December 28, 2018
Summary
Daily use of 7.2% alcohol mouthwash is safe for gingival fibroblasts, aiding recovery after oral surgery. Higher concentrations, however, cause irreversible cell damage.
Area of Science:
- Oral biology
- Cellular toxicology
Background:
- Alcohol is a significant global health risk factor.
- Alcohol-containing mouthwashes may impede oral wound healing.
- Previous research shows varied effects of alcohol concentrations on cells.
Purpose of the Study:
- To investigate the impact of 7.2% and 22% ethanol on human gingival fibroblasts.
- To visualize the direct effects of alcohol on cell morphology and proliferation.
Main Methods:
- Human gingival fibroblasts were cultured in vitro using the PANsystem 2000 device.
- Cells were exposed to 7.2% and 22% ethanol solutions.
- Cellular reactions were observed for 48 hours with continuous imaging.
Main Results:
- Both 7.2% and 22% ethanol negatively impacted cell morphology and proliferation.
- Gingival fibroblasts recovered normal morphology and division after 10 hours exposure to 7.2% ethanol.
- Exposure to 22% ethanol resulted in irreversible cellular damage.
Conclusions:
- 7.2% alcohol concentration in mouthwash is non-toxic to gingival fibroblasts.
- Daily use of 7.2% alcohol mouthwash may be recommended post-periodontal surgery.
- Higher alcohol concentrations (22%) are detrimental to oral fibroblast health.
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