Characterization of ankaflavin from Penicillium aculeatum and its cytotoxic properties
Related Experiment Video
Updated: Jan 31, 2026

Isolation and Characterization of Neutrophils with Anti-Tumor Properties
Published on: June 19, 2015
Soumya Krishnamurthy1, Konappa Narasimha Murthy2, Sharmila Thirumale3
1Department of Microbiology, Field Marshal K. M. Cariappa College, A Constituent College of Mangalore University, Karnataka, India.
Abstract:
The pigment was extracted from Penicillium aculeatum, purified and characterized as Ankaflavin by spectroscopic analysis. The stability of the pigment was determined under various conditions and was found to possess high stability. The cytotoxicity property of the purified pigment was determined by MTT assay in MCF-7, HCT116 and PC-3 and the studies were compared with its activity in CHOK1 cells. In MCF-7 and in CHOK 1 cells, the pigment exhibited very less toxicity. However, significant cytotoxicity was observed in HCT116 and PC-3 cells with IC50 of 162 μg mL-1 and 85 μg mL-1 for HCT116 and PC-3 cells respectively. In vitro toxicity was tested by haemolysis assay and MTT assay in HEK 293 cells. The pigment showed least cytotoxicity (<5%) at 160 and 320 μg mL-1 concentrations HEK 293 cells and negligible (<5%) toxicity on human erythrocytes at 160 and 320 μg mL-1, the highest concentrations tested.
More Related Videos
Related Concept Videos
Physical and Chemical Properties of Matter
Properties of Transition Metals
General Properties of Solutions
Convolution Properties I
The commutative property reveals that the input and the impulse response of an LTI (Linear Time-Invariant) system can be interchanged without affecting the output:
Properties of DTFT II
The frequency differentiation property is illustrated by considering a DTFT pair and differentiating both sides with respect to ω.
Properties of the z-Transform I

