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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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RNA Editing02:23

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Classification of Titrimetric Analysis Based on Reaction Types01:01

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Titrimetric analysis in solution chemistry involves measuring the volume of solutions and is often called volumetric analysis. The standard solution of known concentration in the burette is called the titrant, whereas the solution of unknown concentration in the flask is called the analyte, or titrand. Titrimetric analyses can be classified into four types based on the reactions between the titrant and analyte.
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Lewis Acids and Bases02:33

Lewis Acids and Bases

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In 1923, G. N. Lewis proposed a generalized definition of acid-base behavior in which acids and bases are identified by their ability to accept or to donate a pair of electrons and form a coordinate covalent bond.
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CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
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Web-based design and analysis tools for CRISPR base editing.

Gue-Ho Hwang1, Jeongbin Park2,3, Kayeong Lim4,5

  • 1Department of Chemistry, Hanyang University, Seoul, South Korea.

BMC Bioinformatics
|December 28, 2018
PubMed
Summary
This summary is machine-generated.

Two new web tools, BE-Designer and BE-Analyzer, simplify CRISPR base editing. BE-Designer helps select target DNA sequences, while BE-Analyzer assesses experimental results from next-generation sequencing data securely and efficiently.

Keywords:
Base editingCRISPRGenome editingNGS analysisWeb-based tool

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Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • CRISPR-Cas systems are efficient genome editing tools.
  • CRISPR base editors enable precise DNA substitutions without donor DNA.
  • Dedicated web tools for base editing design and analysis were lacking.

Purpose of the Study:

  • To develop user-friendly web tools for CRISPR base editing.
  • To facilitate the design of base editor target sequences.
  • To enable efficient analysis of next-generation sequencing data from base editing experiments.

Main Methods:

  • Development of BE-Designer for identifying target sequences and off-target sites.
  • Development of BE-Analyzer for processing and visualizing next-generation sequencing data.
  • Client-side processing in BE-Analyzer to enhance speed and data security.

Main Results:

  • BE-Designer provides target sequence options and off-target site information.
  • BE-Analyzer presents mutation data in tables and interactive graphs.
  • Client-side execution of BE-Analyzer reduces upload times and secures large datasets.

Conclusions:

  • BE-Designer and BE-Analyzer are valuable web tools for CRISPR base editing.
  • These tools streamline the design and analysis phases of base editing workflows.
  • The developed tools enhance the practical application of CRISPR base editing technology.