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miRNA-30c can be used as a target in the diagnosis and treatment of osteosarcoma
Rongxin Sun1,2, Aikeremujiang Muheremu3, Yihe Hu1
1Department of Orthopedics, Xiangya Hospital, CentralSouth University, Kaifu District, Changsha, Hunan 86410008, China, dadi136@126.com.
Objective:
Osteosarcoma is a highly malignant osseous sarcoma with poor prognosis. Previous studies indicated that miRNA-30c may play an important role in the development of osteosarcoma, but its mechanism is not yet clear. The current research was carried out to explore the potential applications of miR-30c in the diagnosis and treatment of osteosarcoma.
Materials And Methods:
Real-time PCR and in situ hybridization were used to test the correlation between miR-30c and the onset of osteosarcoma. In vitro transfection of miR-30 mimic was used to test the effect of miR-30c on the development of osteosarcoma. Cell Counting Kit-8, formation of Petri dish clones, in vivo formation of tumor, flow cytometry tests and Transwell analysis were used to assess the effect of miR-330c on the metastatic potential and invasiveness of osteosarcoma.
Results:
Reverse transcriptase-PCR analysis and in situ hybridization tests revealed that the expression of miR-30c was lower in the osteosarcoma tissue than in normal bone tissue (P<0.05). Low expression of miR-30c was associated with advanced osteosarcoma staging and low cellular differentiation. Multivariate analysis revealed that lower expression of miR-30c was associated with shorter survival of patients (P<0.01). U2OS cell growth was significantly inhibited when transfected with miR-30c mimic. Flow cytometry analysis revealed that overexpression of miR-30c could induce apoptosis of osteosarcoma cells. In vitro Petri dish cloning experiment showed that overexpression of miR-30c reduced the cloning ratio of U2OS cells from 21% to 7%. At the same time, overexpression of miR-30c inhibited the formation of sarcoma in nude mice. Transwell experiments indicated that overexpression of miR-30c could reduce the invasiveness of U2OS cells.
Conclusion:
Low expression of miR-30c was associated with high probability of onset and aggressiveness of osteosarcoma and shorter patient survival. Upregulation of miR-30c could downregulate the invasiveness of osteosarcoma. Therefore, miR-30 can be used in the development of future diagnostic and therapeutic techniques.
Insights
Low expression of microRNA-30c (miR-30c) is linked to aggressive osteosarcoma and poor patient survival. Restoring miR-30c levels may inhibit tumor growth and reduce invasiveness, offering potential diagnostic and therapeutic applications.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Osteosarcoma is a malignant bone cancer with a poor prognosis.
- MicroRNA-30c (miR-30c) has been implicated in osteosarcoma development, but its precise role remains unclear.
- Understanding miR-30c's function is crucial for developing novel diagnostic and therapeutic strategies for osteosarcoma.
Purpose of the Study:
- To investigate the role of miR-30c in osteosarcoma.
- To explore the potential of miR-30c as a biomarker for osteosarcoma diagnosis.
- To evaluate miR-30c's therapeutic potential in inhibiting osteosarcoma progression.
Main Methods:
- Real-time PCR and in situ hybridization were employed to assess miR-30c expression levels in osteosarcoma tissues.
- In vitro and in vivo experiments, including cell transfection, proliferation assays, apoptosis analysis, and tumor formation studies, were conducted.
- Transwell assays were utilized to evaluate the impact of miR-30c on osteosarcoma cell invasiveness.
Main Results:
- miR-30c expression was significantly lower in osteosarcoma tissues compared to normal bone tissues.
- Low miR-30c expression correlated with advanced tumor stage, poor cellular differentiation, and reduced patient survival.
- Overexpression of miR-30c inhibited osteosarcoma cell proliferation, induced apoptosis, and reduced tumor growth and invasiveness in vitro and in vivo.
Conclusions:
- Reduced miR-30c expression is associated with increased osteosarcoma onset, aggressiveness, and poorer patient outcomes.
- Upregulating miR-30c demonstrates potential in suppressing osteosarcoma invasiveness.
- miR-30c holds promise for the development of future diagnostic and therapeutic interventions for osteosarcoma.
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