Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Paramagnetic microspheres as immunological markers for light and electron microscopy.

M T Rana1, E C Pearson, C R Barker

  • 1Department of Haematological Medicine, University of Cambridge Clinical School, U.K.

Journal of Immunological Methods
|December 9, 1988
PubMed
Summary

Magnetizable microspheres (Dynabeads) can label cell surfaces for separation. This study shows Dynabeads can also label cells for ultrastructural analysis, bridging light and electron microscopy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Bag-1 inhibitor, Thio-2, reverses an atypical 3D morphology driven by Bag-1L overexpression in a MCF-10A model of ductal carcinoma in situ.

Oncogenesis·2016
Same author

Effect of chronic MK-801 and/or phenytoin on the acquisition of complex behaviors in rats.

Neurotoxicology and teratology·2007
Same author

Behavioral effects associated with chronic ketamine or remacemide exposure in rats.

Neurotoxicology and teratology·2007
Same author

Assessing the potential toxicity of MK-801 and remacemide: chronic exposure in juvenile rhesus monkeys.

Neurotoxicology and teratology·2002
Same author

Differential effects of two NMDA receptor antagonists on cognitive-behavioral development in nonhuman primates I.

Neurotoxicology and teratology·2001
Same author

Differential effects of two NMDA receptor antagonists on cognitive--behavioral performance in young nonhuman primates II.

Neurotoxicology and teratology·2001

Area of Science:

  • Cell biology
  • Microscopy techniques
  • Immunotechnology

Background:

  • M-450 Dynabeads are magnetizable polystyrene microspheres used for cell surface labeling and separation.
  • Antibodies of the IgM isotype can be adsorbed onto Dynabeads for specific cell targeting.
  • Current applications primarily involve light microscopy and cell separation via magnetic fields.

Purpose of the Study:

  • To investigate the utility of Dynabeads for ultrastructural cell labeling.
  • To demonstrate Dynabeads as a tool bridging light and electron microscopy.
  • To establish optimal fixation methods for preserving Dynabead-labeled cells at the ultrastructural level.

Main Methods:

  • Utilized M-450 Dynabeads coated with novel monoclonal antibodies (CH-F42, CH-E25).

Related Experiment Videos

  • Applied Dynabeads to label cell lines K562 and U937.
  • Developed a rapid, gentle fixation protocol using buffered glutaraldehyde and ethanolamine for ultrastructural preservation.

    • Main Results:

    • Successfully demonstrated ultrastructural labeling of cells using antibody-coated Dynabeads.
    • Observed preservation of specific rosettes without artifactual aggregates.
    • Validated the method on K562 and U937 cell lines.

    Conclusions:

    • Dynabeads can be effectively used for ultrastructural cell labeling, extending their application beyond light microscopy.
    • The developed fixation technique preserves cellular integrity and specific labeling for electron microscopy.
    • Dynabeads offer a valuable tool for correlative light and electron microscopy studies.