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Updated: Jan 31, 2026

DNA-based Fish Species Identification Protocol
Published on: April 28, 2010
Three new species of EntolomasubgenusPouzarella from China based on morphological and molecular data
Xiao-Lan He1, Egon Horak2, Di Wang1
1Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences Chengdu China.
Abstract:
In the present paper, three additional species of EntolomasubgenusPouzarella viz. E.erectoides, E.griseocarpum and E.rubropilosum are described from China. E.rubropilosum is a typical species in section Pouzarella; E.griseocarpum and E.erectoides are members of sect. Dysthales. The taxa are further confirmed by ITS, RPB2, LSU and mtSSU analyses and phylogenetic relationships with other Entolomasubgen.Pouzarella species are also discussed. ITS sequence analysis showed that the sizes of the entire ITS region and ITS1 are remarkably divergent, while the ITS2 is conserved in length within Entolomasubgen.Pouzarella. Molecular analyses, based on the combined dataset, demonstrated that species diversity of subgen.Pouzarella in China is much higher than previously thought, in the present study twenty phylogenetic species from China are taken into consideration. On the other hand, morphological and molecular analyses suggested that classification of Entolomasubgen.Pouzarella probably has to be fundamentally re-adjusted based on additional data.
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