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Updated: Jan 31, 2026

Fabricating Reactive Surfaces with Brush-like and Crosslinked Films of Azlactone-Functionalized Block Co-Polymers
Published on: June 30, 2018
Biomolecule Sensor Based on Azlactone-Modified Hydrogel Films
Jie Li1, Xiaoqian Yu1, Artjom Herberg1
1Department Chemie, Universität Paderborn, Warburgerstraße 100, D-33098, Paderborn, Germany.
Abstract:
A 3D hydrogel layer is probed by combining surface plasmon resonance with optical waveguide spectroscopy to detect biomolecules. A template terpolymer P(DMAAm-co-DMIAAm-co-VDMA) is synthesized via reversible addition-fragmentation chain-transfer polymerization. The terpolymer is then modified with an amino group bearing biotin to enable biomolecular recognition for streptavidin. A hydrogel thin layer is prepared onto a gold surface after spin-coating and photo-crosslinking of the modified polymer. Finally, the hydrogel is utilized to quantitatively detect streptavidin by using surface plasmon resonance-optical waveguide spectroscopy measurements.
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