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Complete development of Caryospora bigenetica (Apicomplexa: Eimeriidae) in vitro
C A Sundermann1, D S Lindsay, R E Tibbs
1Department of Zoology and Wildlife Science, Auburn University, Alabama 36849.
The Journal of Protozoology
|November 1, 1988
Summary
This study details the in vitro development of Caryospora bigenetica, a coccidian parasite. Researchers successfully cultured the parasite in host cells, observing key life cycle stages and confirming infectivity of in vitro-derived stages.
Area of Science:
- Parasitology
- Cell Biology
- Infectious Diseases
Background:
- Caryospora bigenetica is a coccidian parasite with a complex life cycle.
- Understanding its in vitro development is crucial for studying host-parasite interactions and developing control strategies.
Purpose of the Study:
- To describe the in vitro development of Caryospora bigenetica using light microscopy.
- To assess the infectivity of in vitro-derived parasite stages.
Main Methods:
- Sporozoites from snake-derived oocysts were inoculated onto primary cotton rat testicle cells, cotton rat kidney cells, and human fetal lung cells.
- Parasite development was monitored daily using light microscopy.
- Infectivity of in vitro-derived merozoites and oocysts was tested in mice and cotton rats, respectively.
Main Results:
- Intracellular sporozoites were observed within 1-2 days post-inoculation (DPI).
- First-generation merozoites appeared at 3 DPI, second-generation at 5 DPI.
- Mature gamonts and unsporulated oocysts formed by 9-10 DPI, with sporulation and excystation observed in vitro.
- In vitro-derived merozoites successfully infected mice, and sporulated oocysts caused facial swelling in cotton rats.
Conclusions:
- The study successfully established and characterized the in vitro development of Caryospora bigenetica.
- In vitro-derived parasite stages retained infectivity, validating the model system for further research.