Related Experiment Video
Updated: Jan 31, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
poreTally: run and publish de novo nanopore assembler benchmarks
Carlos de Lannoy1, Judith Risse2, Dick de Ridder1
1Bioinformatics Group, WUR, 6700 AP Wageningen, The Netherlands.
Summary:
Nanopore sequencing is a novel development in nucleic acid analysis. As such, nanopore-sequencing hardware and software are updated frequently and extensively, which quickly renders peer-reviewed publications on analysis pipeline benchmarking efforts outdated. To provide the user community with a faster, more flexible alternative to peer-reviewed benchmark papers for de novo assembly tool performance we constructed poreTally, a comprehensive benchmarking tool. poreTally automatically assembles a given read set using several often-used assembly pipelines, analyzes the resulting assemblies for correctness and continuity, and finally generates a quality report, which can immediately be published on Github/Gitlab.
Availability And Implementation:
poreTally is available on Github at https://github.com/ cvdelannoy/poreTally, under an MIT license.
Supplementary Information:
Supplementary data are available at Bioinformatics online.
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