Phosphorus retention in corn, spray dried plasma protein, soybean meal, meat and bone meal, and canola meal using a precision-fed rooster assay
Related Experiment Video
Updated: Jan 31, 2026

Meal Duration as a Measure of Orofacial Nociceptive Responses in Rodents
Published on: January 10, 2014
J A Munoz1, C D Hanna1, P L Utterback1
1Department of Animal Sciences, University of Illinois at Urbana-Champaign, IL 61801, USA.
Abstract:
Four experiments were conducted to evaluate the precision-fed rooster assay for determining excreta phosphorus retention values for 5 feed ingredients in which roosters were precision-fed the ingredients and excreta were collected quantitatively for 48 h. The first rooster assay determined the effects of increasing P intakes on excreta P retention values for corn. This assay involved feeding either 20 g of corn or 20 g of corn supplemented with increasing amounts of KH2PO4 to provide total P intakes of 51 to 351 mg and non-phytate P intakes of 16 to 316 mg. The excreta P retention value for corn fed alone was 75% but decreased greatly when non-phytate P intakes were 76 mg or higher for the corn diets containing added KH2PO4. The second precision-fed rooster assay involved feeding increasing amounts of spray dried plasma protein (SDPP) (5 to 20 g) which provided non-phytate P intakes of 61 to 242 mg. Excretion of P increased and excreta P retention values decreased from 94 to 60% as SDPP intake increased from 5 to 20 g. Experiment 3 determined excreta P retention values for solvent extracted dehulled soybean meal (SBM) (24 g intake) and also the effect of increasing intakes of SDPP (5 to 10 g) and meat and bone meal (MBM) (1.5 to 10 g) on their excreta P retention values. The excreta P retention value for SBM was 41%. Excreta P retention values for SDPP again decreased as P intake increased. Excreta P retention values for MBM were low (27 to 35%) at all intakes. In Experiment 4, roosters were tube-fed 8, 16, or 24 g of canola meal and excreta P retention values varied from 23 to 35% among intake levels. The results of this study indicated that excreta P retention values often varied greatly among different levels of ingredient and non-phytate phosphorus intake and suggest that the precision-fed assay may be useful for determining bioavailability of P only if non-phytate P intakes are low. In addition, the assay may not be accurate for ingredients which contain high Ca levels such as MBM.
Related Concept Videos
The Phosphorus Cycle
Drug Distribution: Plasma Protein Binding
Protein Buffers in Blood Plasma and Cells
Certain amino acids can exist in a zwitterion state at a...
Types of Hormones
Kinetic Energy
Chirality at Nitrogen, Phosphorus, and Sulfur
A consequence of chirality is the need for enantiomeric resolution. While this is theoretically possible for all...

