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Updated: Jan 31, 2026

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
A Novel Chemiluminescent Probe Based on 1,2-Dioxetane Scaffold for Imaging Cysteine in Living Mice
Jinyu Sun1, Zhian Hu1, Sichun Zhang1
1Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation , Tsinghua University , Beijing 100084 , China.
Abstract:
A novel chemiluminescent probe for detection of cysteine (Cys) from other biothiols has been reported by utilizing the excellent chemiluminescent Schaap's adamantylidene-dioxetane scaffold. After careful assessment, the probe CL-Cys could detect Cys with high sensitivity and total light photons increased by 28-fold after the probe was treated with Cys, with the detection limit of 7.5 × 10-8 M. Finally, CL-Cys was further utilized for the chemiluminescent imaging of endogenous Cys in a living mouse. In general, this probe has a remarkable ability to detect Cys, which provides a valuable method for interrogation of the Cys roles in more biological and pathological processes.
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