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[Testicular injury induced by DBP involved in activation of ERK pathway in KM mice]

Juan Li1, Zhe Wu1, Jian Cheng1

  • 1Department of Internal Medicine, College of Clinical Medicine, Hubei University of Science and Technology, Xianning 437100, China.

Wei Sheng Yan Jiu = Journal of Hygiene Research
|December 30, 2018
PubMed
Abstract

Insights

Dibutyl phthalate (DBP) exposure causes testicular injury in mice by increasing oxidative stress and activating the ERK pathway. Vitamin E and nimodipine treatments partially reversed these effects, indicating potential therapeutic targets.

Area of Science:

  • Toxicology
  • Reproductive Biology
  • Cell Signaling

Background:

  • Environmental toxicants like dibutyl phthalate (DBP) pose risks to reproductive health.
  • Extracellular regulated protein kinase (ERK) pathway dysregulation is implicated in various cellular injuries.

Purpose of the Study:

  • To investigate the role of extracellular regulated protein kinase (ERK) pathway activation in dibutyl phthalate (DBP)-induced testicular injury in mice.
  • To evaluate the protective effects of vitamin E (VE) and nimodipine (Ni) against DBP-induced testicular damage.

Main Methods:

  • Male KM mice were administered DBP, VE, Ni, or combinations thereof for 28 days.
  • Evaluated body weight, testis weight, organ coefficient, sperm density, and testicular histomorphology.
  • Measured reactive oxygen species (ROS), malondialdehyde (MDA), calmodulin (CaM), and phosphorylated ERK (p-ERK) levels.

Main Results:

  • DBP exposure significantly decreased body weight, testis weight, organ coefficient, and sperm density while increasing testicular injury, ROS, MDA, and p-ERK levels.
  • DBP exposure also decreased CaM levels.
  • Co-administration of VE and Ni partially ameliorated DBP-induced testicular damage, reducing ROS and MDA, and normalizing CaM and p-ERK levels.

Conclusions:

  • DBP induces testicular injury in mice, potentially via oxidative stress and Ca2+ signaling pathways that activate the ERK1/2 pathway.
  • Vitamin E and nimodipine demonstrate protective effects against DBP-induced testicular damage, suggesting therapeutic potential.
Keywords:
antagonistdibutyl phthalateextracellular regulated protein kinaseoxidative stresstestis

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