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Updated: Jan 31, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Measuring Hepatitis C Virus Envelopment by Using a Proteinase K Protection Assay
Allison E Roder1, Stacy M Horner2,3
1Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, USA.
Abstract:
The infectious virion of hepatitis C virus (HCV) is made up of the viral nucleocapsid surrounded by an envelope that contains an ER-derived membrane bilayer, cellular lipids, and the viral E1 and E2 glycoproteins. Because the infectious HCV particle contains both protein and lipid layers, selective disruption of these layers and analysis for the presence or absence of resulting virion components can be used to study the virion assembly process. This chapter describes an experimental method to measure HCV virion envelopment, which can reveal the mechanisms of how specific viral protein-protein interactions and host factors contribute to the process of HCV envelopment.
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