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Related Experiment Video

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Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells
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Investigating Hepatitis C Virus Infection Using Super-Resolution Microscopy.

Pedro Matos Pereira1, Caron Jacobs1, Joe Grove2

  • 1Quantitative Imaging and Nanobiophysics Group, MRC Laboratory for Molecular Cell Biology, Department of Cell and Developmental Biology, University College London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|December 30, 2018
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Super-resolution microscopy (SRM) reveals nanoscale virus replication events. This protocol details imaging hepatitis C virus-infected cells using localization SRM for broader accessibility.

Keywords:
HepatitisImagingMicroscopySuper-resolutionVirus

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Area of Science:

  • Cell biology
  • Microscopy
  • Virology

Background:

  • Super-resolution microscopy (SRM) offers unprecedented nanoscale insights into cellular processes.
  • Understanding virus replication at the nanoscale is crucial for developing effective antiviral strategies.

Purpose of the Study:

  • To present a generalized protocol for imaging hepatitis C virus (HCV)-infected cells using localization SRM.
  • To make advanced super-resolution imaging techniques accessible to researchers new to the field.

Main Methods:

  • Detailed sample preparation techniques for HCV-infected cells.
  • Immunostaining protocols optimized for super-resolution imaging.
  • Guidelines for data collection and super-resolution image reconstruction.

Main Results:

  • A comprehensive protocol enabling visualization of nanoscale events during HCV replication.
  • Demonstration of the applicability of localization SRM to virology research.

Conclusions:

  • The developed protocol facilitates the study of virus replication dynamics at the nanoscale.
  • This work lowers the barrier for researchers to adopt SRM for virological studies.