Structural characterization of lignin and its carbohydrate complexes isolated from bamboo (Dendrocalamus sinicus)

Isolation of earth abundant biopolymer, Lignin, from Dendrocalamus sinicus and their structural properties were investigated to achieve its large-scale practical applications in value-added products. Two lignin fractions (MWL, DSL) were isolated with successive treatments of dioxane and dimethylsulfoxide (DMSO) from dewaxed and ball milled bamboo (D. sinicus) sample. The two-step treatments yielded 52.1% lignin based on the total lignin content in the dewaxed bamboo sample. Spectroscopy analyses indicated that the isolated bamboo lignin was a typical grass lignin, consisting of p-hydroxyphenyl, guaiacyl, and syringyl units. The major interunit linkages presented in the obtained bamboo lignin were β-O-4' aryl ether linkages, together with lower amounts of β-β', β-5', and β-1' linkages. The tricin was detected to be linked to lignin polymer through the β-O-4' linkage in the bamboo. In addition, phenyl glycoside and benzyl ether lignin-carbohydrate complexes (LCC) linkages were clearly detected in bamboo (D. sinicus), whereas the γ-ester LCC linkages were ambiguous due to the overlapping NMR signals with other substructures. The detailed structural properties of the obtained lignin fraction together with the light-weight will benefit efficient utilization of natural polymers as a possibly large-scale bio-based precursor for making polymeric materials, biochemicals, functional carbon and biofuels, and multifunctional polymer nanocomposites.