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Updated: Jan 31, 2026

The Use of an Automated System GreenFeed to Monitor Enteric Methane and Carbon Dioxide Emissions from Ruminant Animals
Published on: September 7, 2015
Reducing enteric methane emissions from dairy cattle: Two ways to supplement 3-nitrooxypropanol
D Van Wesemael1, L Vandaele2, B Ampe2
1Animal Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food, Scheldeweg 68, 9090 Melle, Belgium; Department of Animal Sciences and Aquatic Ecology, Laboratory for Animal Nutrition and Animal Product Quality, Ghent University, Coupure Links 653 Block F, 9000 Ghent, Belgium.
3-nitrooxypropanol (3-NOP) effectively reduces enteric methane emissions in dairy cows. Whether mixed with roughage or in concentrate pellets, 3-NOP offers a viable strategy for lowering greenhouse gas output from cattle.
Area of Science:
- Animal Science
- Agricultural Science
- Environmental Science
Background:
- Enteric methane (CH4) emissions from dairy cows contribute significantly to greenhouse gas output.
- Reducing methane emissions is crucial for the sustainability of the dairy sector.
- 3-nitrooxypropanol (3-NOP) is a promising feed additive for mitigating enteric methane.
Purpose of the Study:
- To evaluate the efficacy of 3-nitrooxypropanol (3-NOP) in reducing enteric methane (CH4) emissions from lactating dairy cows.
- To compare the effects of incorporating 3-NOP into roughage versus a concentrate pellet on CH4 emissions and cow performance.
- To assess the impact of 3-NOP supplementation on dry matter intake, milk production, and milk composition.
Main Methods:
- Thirty Holstein Friesian cows were divided into three groups: control (no additive), 3-NOP mixed with roughage (NOPbas), and 3-NOP in concentrate pellets (NOPconc).
- Cows received 1.6 g of 3-NOP/cow/day for 10 weeks, following a 2-week pretreatment and preceding a 2-week washout period.
- Methane emissions were measured using a GreenFeed unit throughout the experimental period.
Main Results:
- Both NOPbas and NOPconc groups showed significant reductions in CH4 production (28% and 23% lower, respectively) compared to the control.
- Methane yield and intensity were reduced by 21-24% in the 3-NOP supplemented groups.
- No significant differences in dry matter intake, milk yield, or milk composition were observed between the treatment groups.
Conclusions:
- 3-nitrooxypropanol (3-NOP) effectively reduces enteric methane emissions in lactating dairy cows.
- Incorporating 3-NOP into concentrate pellets is as effective as mixing it with roughage for methane reduction.
- 3-NOP offers a flexible and effective tool for mitigating greenhouse gas emissions in the dairy industry without compromising animal performance.
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