Keratin nanoparticles-coating electrospun PVA nanofibers for potential neural tissue applications

Keratin has the potential to improve biocompatibility and bioactivity of polymeric nanofibers. However, the addition of keratin into the blend nanofiber would decrease the mechanical properties of nanofibers due to the poor spinnability of keratin, and caused inhomogeneous distribution of keratin inside the nanofibers. Therefore, polymeric nanofibers surface-modified with keratin nanoparticles would improve the hydrophility and mechanical property. In this study, keratose (oxidative keratin, KOS) nanoparticles-coating PVA nanofibers (KNPs/PVA) were fabricated by electrospray deposition after electrospinning and acted on neural cells. The chemical conformation, mechanical properties and wettability of KNPs/PVA nanofibers were characterized. The KNPs/PVA nanofibers provided better wettability and stronger mechanical properties compared to KOS/PVA blend nanofibers at the same mass ratio of KOS to PVA. Furthermore, KNPs/PVA nanofibers displayed better cyto-biocompatibility in terms of cell morphology, adhesion and proliferation compared with PVA nanofibers and KOS/PVA blend nanofibers. These results suggested that polymeric nanofibers surface-modified with KOS nanoparticles can provide superior wettability, mechanical properties and biocompatibility by comparison with the blend nanofibers.