Genotoxicity of textile dye C.I. Disperse Blue 291 in mouse bone marrow
Fábio Henrique Fernandes1, Gisela de Aragão Umbuzeiro2, Daisy Maria Fávero Salvadori3
1UNESP - São Paulo State University, Medical School, Department Pathology - Rubião Júnior, 18.618-687, Botucatu, São Paulo, Brazil.
Abstract:
Color Index (C.I.) Disperse Blue 291 (DB291) is an azo dye used by the textile industry. After yarn dyeing, wastewater containing the dye, released into the aquatic environment, may pollute drinking water sources. We investigated the mutagenicity and genotoxicity of DB291 in male Swiss mice, following oral administration. Micronucleated cells, primary DNA damage (comet assay) in blood, liver, and kidney cells, and BAX, BCL2, SMAD4 and TNFA gene expression in leukocytes were evaluated. An increased frequency of micronucleated polychromatic erythrocytes (MNPCEs) was observed in animals treated with 50 mg/kg bw; no other genetic alteration was detected. Neither primary DNA damage nor changes in gene expression were observed.
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