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Updated: Jan 31, 2026
Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis
Published on: May 16, 2025
Relevance of Helicobacter pylori infection in Egyptian multiple sclerosis patients
Steven Emil Gerges1, Taha Kamel Alosh1, Salma Hamed Khalil1
1Faculty of Medicine, Ain Shams University, 19 Awad Fahmy st-El Zatoun, Cairo, Egypt.
Background:
Multiple sclerosis (MS) is an autoimmune demyelinating disorder. The etiology of MS remains unknown exactly. Helicobacter pylori heat shock proteins were suggested as a potential trigger of immune system causing MS.
Objectives:
The aim of this study was to assess the level of anti-Helicobacter pylori heat shock proteins 60 (Hp hsp60) antibodies at patients of MS and to correlate it with various epidemiological and clinical data.
Subjects And Methods:
This study design was a cross-sectional case control one. A total of 65 patients with multiple sclerosis diagnosed according to 2010 revised McDonald criteria and other 65 age- and sex-matched healthy controls were included in this study. All participants were subjected to full history taking, complete neurological examination including Expanded Disability Status Scale (EDSS) for the patients, measurement of serum level of anti-Hp hsp60 IgG using ELISA technique, and MRI brain for all the patients, being a goldstone for inclusion in the study.
Results:
There was statistically significant high level of anti-Hp hsp60 IgG at MS patients especially secondary progressive multiple sclerosis (SPMS) patients. Moreover, a positive statistically significant correlation was found between it and age of patients, duration of illness, and EDSS.
Conclusion:
We conclude that hsp60 of Hp may be a useful biomarker for attesting course progression in MS.
Insights
Elevated anti-Helicobacter pylori heat shock protein 60 (Hp hsp60) antibodies were found in multiple sclerosis (MS) patients, particularly those with secondary progressive MS. This suggests Hp hsp60 may indicate MS progression.
Area of Science:
- Neuroimmunology
- Autoimmune Diseases
- Microbial Immunology
Background:
- Multiple sclerosis (MS) is an autoimmune demyelinating disorder with an unknown exact etiology.
- Heat shock proteins from Helicobacter pylori (Hp hsp60) have been implicated as potential immune triggers for MS.
Purpose of the Study:
- To quantify anti-Hp hsp60 IgG antibody levels in MS patients.
- To correlate these antibody levels with epidemiological and clinical factors in MS.
Main Methods:
- Cross-sectional case-control study involving 65 MS patients and 65 healthy controls.
- Assessment included medical history, neurological examination (EDSS), serum anti-Hp hsp60 IgG via ELISA, and brain MRI.
Main Results:
- Significantly higher levels of anti-Hp hsp60 IgG were observed in MS patients, especially those with secondary progressive MS (SPMS).
- A positive correlation was found between anti-Hp hsp60 IgG levels and patient age, disease duration, and Expanded Disability Status Scale (EDSS) scores.
Conclusions:
- Hp hsp60 may serve as a valuable biomarker for monitoring disease progression in multiple sclerosis.
- Further research is warranted to elucidate the role of Hp hsp60 in MS pathogenesis.
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