Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

6.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
6.6K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

3.5K
3.5K
Regulated mRNA Transport02:22

Regulated mRNA Transport

7.0K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
7.0K
pre-mRNA Processing02:01

pre-mRNA Processing

57.5K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
57.5K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

8.8K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.8K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

8.2K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Age- and sex-dependent transcriptomic network alterations in sepsis.

npj aging·2026
Same author

Strength of Omics in Uncovering Sepsis Mechanisms-A Perspective.

Current health sciences journal·2026
Same author

Challenges in Diagnosis and Management of Coffin-Lowry Syndrome-Single-Center Experience.

Diagnostics (Basel, Switzerland)·2026
Same author

Ferritin and Liver Steatosis in Children: Interactions Between Metabolic Clustering and <i>PNPLA3</i> Variants.

International journal of molecular sciences·2026
Same author

Phenotypic Spectrum in Three Romanian Patients with 8q23-q24 Deletions.

International journal of molecular sciences·2026
Same author

The CrescNet Registry Achondroplasia Module: Real-World Demographic Data and Clinical Outcomes in Untreated and Vosoritide-Treated Individuals.

Hormone research in paediatrics·2026

Related Experiment Video

Updated: Jan 31, 2026

Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer
06:49

Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer

Published on: February 10, 2023

2.8K

Interleukin-8 mRNA Expression in Locally Advanced Colorectal Cancer Patients.

A Bărbălan1, Ioana Streața2, Elena Tatiana Ivan3

  • 1PhD student, University of Medicine and Pharmacy Craiova, Craiova, Romania.

Current Health Sciences Journal
|January 1, 2019
PubMed
Summary
This summary is machine-generated.

Interleukin-8 (IL-8) gene expression was lower in colorectal tumors compared to adjacent normal tissues. Further research is needed to confirm these findings in larger patient groups.

Keywords:
Interleukin-8 mRNAadvanced colorectal cancerendoscopypreoperative analysis

More Related Videos

Computer-Aided Three-Dimensional Visualization in the Treatment of Locally Advanced Thyroid Cancer
03:55

Computer-Aided Three-Dimensional Visualization in the Treatment of Locally Advanced Thyroid Cancer

Published on: June 9, 2023

1.0K
Competing-Risk Nomogram for Predicting Cancer-Specific Survival in Multiple Primary Colorectal Cancer Patients after Surgery
06:46

Competing-Risk Nomogram for Predicting Cancer-Specific Survival in Multiple Primary Colorectal Cancer Patients after Surgery

Published on: September 27, 2024

911

Related Experiment Videos

Last Updated: Jan 31, 2026

Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer
06:49

Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer

Published on: February 10, 2023

2.8K
Computer-Aided Three-Dimensional Visualization in the Treatment of Locally Advanced Thyroid Cancer
03:55

Computer-Aided Three-Dimensional Visualization in the Treatment of Locally Advanced Thyroid Cancer

Published on: June 9, 2023

1.0K
Competing-Risk Nomogram for Predicting Cancer-Specific Survival in Multiple Primary Colorectal Cancer Patients after Surgery
06:46

Competing-Risk Nomogram for Predicting Cancer-Specific Survival in Multiple Primary Colorectal Cancer Patients after Surgery

Published on: September 27, 2024

911

Area of Science:

  • Oncology
  • Molecular Biology
  • Gastroenterology

Background:

  • Interleukin-8 (IL-8) is implicated in promoting malignant tumor progression and regulating angiogenesis.
  • Understanding IL-8's role in colorectal cancer (CRC) is crucial for developing targeted therapies.

Purpose of the Study:

  • To quantify and compare the gene expression levels of IL-8 in colorectal tumors (CCR) versus adjacent peritumoral tissue.
  • To investigate the differential expression of IL-8 in cancerous and non-cancerous colorectal tissue.

Main Methods:

  • Colorectal tumor and adjacent normal mucosa samples were collected via endoscopic biopsy from CRC patients.
  • Quantitative Real-Time PCR (qRT-PCR) was employed to assess IL-8 mRNA expression levels in both tissue types.

Main Results:

  • IL-8 gene expression was found to be significantly higher in adjacent normal mucosa compared to tumoral tissue across all samples analyzed.
  • This suggests a potential downregulation of IL-8 in colorectal tumor tissues.

Conclusions:

  • The study observed lower IL-8 expression in colorectal tumors than in surrounding normal tissues.
  • Larger cohort studies are recommended to validate these preliminary findings and explore their clinical implications.