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Isolation of Collagen from Marine Resources from the Black Sea
Melat Cherim1, Rodica Sirbu1, Ionela Belu1
1PhD-Student Pharmacyst, Doctoral School "Carol Davila" University of Medicine and Pharmacy, Faculty of Pharmacy, Bucharest, Romania.
Purpose:
Evaluation of possibilities of obtaining type I fibrillar collagen from fish skin using the Black Sea marine resources and suitable cross-linking agent for this one to stay stable at body or room temperature.
Materials And Methods:
Collagen has been extracted from the skin of the Grey mullet and Sturgeon by the acidic method with Acetic acid 0.5M. Cross-linking has been performed by using Tanic acid at 4-6°C. The rheological behaviour was determined by using a Haake VT 550 rheo-viscometer.
Results:
The collagen was isolated from the skin of Gray mullet and Sturgeon. (Acipenser gueldenstaedtii). Hydrogels have a pseudoplastic behavior. Tanic acid was used for cross-linking like a better alternative, eliminating the toxicity of glutaraldehyde.
Conclusion:
The yield of collagen extraction was higher for the Gray mullet skin, than Sturgeon. Pseudo-plastic behavior allows them to be successfully applied in the treatment of wounds. The isolated type I collagen may serve as an attractive alternative to mammalian collagen for biomedical and pharmaceutical applications.
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