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Updated: Jan 31, 2026

Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning
Published on: April 15, 2010
High-resolution magic angle spinning NMR studies for metabolic characterization of Arabidopsis thaliana mutants with
Dieuwertje Augustijn1, Niels van Tol2, Bert J van der Zaal2
1Leiden Institute of Chemistry, Leiden University, RA Leiden, The Netherlands.
Abstract:
Developing smart crops which yield more biomass to meet the increasing demand for plant biomass has been an active area of research in last few decades. We investigated metabolic alterations in two Arabidopsis thaliana mutants with enhanced growth characteristics that were previously obtained from a collection of plant lines expressing artificial transcription factors. The metabolic profiles were obtained directly from intact Arabidopsis leaves using high-resolution magic angle spinning (HR-MAS) NMR. Multivariate analysis showed significant alteration of metabolite levels between the mutants and the wild-type Col-0. Interestingly, most of the metabolites that were reduced in the faster-growing mutants are generally involved in the defence against stress. These results suggest a growth-defence trade-off in the phenotypically engineered mutants. Our results further corroborate the idea that plant growth can be enhanced by suppressing defence pathways.
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