Yeast flocculation: a dynamic equilibrium

The steady state in yeast flocculation is a dynamic equilibrium between flocculated and dispersed yeast cells. The free cell concentration is directly proportional to the total cell concentration and may be expressed as an equilibrium constant. Increased agitation decreases floc size and equilibrium constant whilst increasing floc-surface area and free-cell concentration. Values of equilibrium constant are influenced by agitation in a complex relationship probably involving the floc-surface area and floc momentum. Inhibition of flocculation by mannose and low pH is reversible and becomes greater with increased agitation. Both these inhibitions appear consistent with a weakening of flocculent bond strength by these inhibitors.