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Related Experiment Videos

Yeast flocculation: a dynamic equilibrium.

M Stratford1, H P Coleman, M H Keenan

  • 1AFRC Institute of Food Research, Norwich Laboratory, U.K.

Yeast (Chichester, England)
|September 1, 1988
PubMed
Summary
This summary is machine-generated.

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Yeast flocculation reaches a dynamic equilibrium. Increased agitation reduces yeast floc size and stability, while mannose and low pH reversibly weaken flocculent bonds.

Area of Science:

  • Microbiology
  • Biophysics

Background:

  • Yeast flocculation is a crucial phenomenon in brewing and biotechnology.
  • It involves a dynamic equilibrium between aggregated (flocculated) and single (dispersed) yeast cells.

Purpose of the Study:

  • To investigate the factors influencing the steady state of yeast flocculation.
  • To understand the impact of agitation, pH, and mannose on yeast cell aggregation.

Main Methods:

  • Studied yeast cell suspensions under varying agitation speeds.
  • Quantified free cell concentration and floc characteristics.
  • Assessed the effects of mannose and low pH on flocculation.

Main Results:

  • Steady state is a dynamic equilibrium with an equilibrium constant related to free cell concentration.

Related Experiment Videos

  • Increased agitation decreased floc size and equilibrium constant, but increased surface area and free cells.
  • Mannose and low pH reversibly inhibited flocculation, with inhibition increasing with agitation.
  • Conclusions:

    • Agitation influences yeast flocculation through a complex relationship involving floc size, surface area, and momentum.
    • Inhibitory effects of mannose and low pH suggest a weakening of the bonds holding yeast flocs together.