Related Experiment Video
Updated: Jan 31, 2026

Isolation and Characterization of RNA-Containing Exosomes
Published on: January 9, 2012
Isolation and characterization of microsatellite loci from Pterocarya stenoptera (Juglandaceae)
Peng-Fei Wang1, Yong Li1, Zhi-Hao Qian1
1Innovation Platform of Molecular Biology College of Forestry Henan Agricultural University Zhengzhou 450002 People's Republic of China.
Premise Of The Study:
Microsatellite markers of Pterocarya stenoptera (Juglandaceae) were developed for future studies on the population genetic diversity and spatial genetic structure of the species.
Methods And Results:
Based on Illumina sequencing of the transcriptome of P. stenoptera, a total of 2452 microsatellites were identified from 83,674 assembled unigenes. One hundred microsatellites were randomly selected to design amplification primer pairs. Of these, 15 were successfully amplified and displayed polymorphism. For these markers, the number of alleles per locus and population ranged from one to six. The levels of observed and expected heterozygosity varied from 0.000 to 1.000 and 0.000 to 0.718, respectively. Furthermore, all of the 15 loci were successfully cross-amplified in another congeneric species (P. hupehensis) and were demonstrated to be polymorphic.
Conclusions:
The microsatellite loci described here can be used for future population genetic and landscape genetic studies on P. stenoptera.
Related Concept Videos
Root Loci for Positive-Feedback Systems
The construction rules for the root locus in positive feedback systems are similar to those in...
DNA Isolation
DNA Isolation
Overview Of Cell Separation And Isolation
Cyclic Processes And Isolated Systems
In the case of a non-isolated system, the change in the internal energy is zero only if the process is cyclic. A thermodynamic process is considered cyclic if the system undergoes a series of changes and returns to its initial state.
Consider a cyclic process that returns to its initial state, undergoing a four-step process. The heat transfer along each...
Techniques for Isolation of Pure Cultures

