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Updated: Jan 31, 2026

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence
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TCF3-activated LINC00152 exerts oncogenic role in osteosarcoma through regulating miR-1182/CDK14 axis

Longlong Zheng1, Nan Hu2, Xiaozhong Zhou3

  • 1Department of Orthopedics, The Second Affiliated Hospital of Suzhou University, Suzhou, Jiangsu, 215008, China; Department of Orthopedics, Chuzhou Medical College of Anhui Medical University, Chuzhou, Anhui, 239000, China.

Pathology, Research and Practice
|January 3, 2019
PubMed

Insights

Long noncoding RNA LINC00152 promotes osteosarcoma progression. This study reveals LINC00152, activated by TCF3, drives cancer cell proliferation and migration via the miR-1182/CDK14 pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in cancer development.
  • The specific role of lncRNA LINC00152 in osteosarcoma (OS) is not well understood.

Purpose of the Study:

  • To investigate the function and mechanism of lncRNA LINC00152 in osteosarcoma.
  • To elucidate the regulatory pathway involving LINC00152, TCF3, and the miR-1182/CDK14 axis in OS.

Main Methods:

  • Quantitative analysis of LINC00152 expression in OS tissues and cell lines.
  • Cell proliferation assays (MTT, colony formation) and migration/invasion assays (Transwell).
  • Chromatin immunoprecipitation (ChIP), luciferase reporter assays, and mechanistic studies to identify regulatory interactions.

Main Results:

  • LINC00152 expression is significantly upregulated in osteosarcoma.
  • Knockdown of LINC00152 inhibits osteosarcoma cell proliferation, migration, and invasion.
  • LINC00152 is transcriptionally activated by TCF3 and functions as a competing endogenous RNA (ceRNA) by sponging miR-1182, thereby upregulating CDK14.

Conclusions:

  • LINC00152 plays a crucial oncogenic role in osteosarcoma.
  • TCF3-induced LINC00152 promotes osteosarcoma progression through the LINC00152/miR-1182/CDK14 axis.
Keywords:
CDK14LINC00152OsteosarcomaTCF3miR-1182

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