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CD147 as an apoptosis regulator in spermatogenesis: deciphering its association with matrix metalloproteinases'
Rezvan Asgari1, Kamran Mansouri1, Mitra Bakhtiari2,3
1Medical Biology Research Center (MBRC), Kermanshah University of Medical Sciences, Kermanshah, Iran.
Abstract:
CD147 plays an important role in germ cells migration and survival/apoptosis during the spermatogenesis process. However, to best of our knowledge, there is no report on the exact role of CD147 gene in the regulation of germ cells apoptosis through matrix metalloproteinases (MMPs). So, the current study aims to evaluate the role of CD147 gene expression in the regulation of germ cells apoptosis in conjunction with MMPs. Real-Time PCR was applied to investigate the expression of CD147, MMP2, MMP7, and MMP9 genes in the azoospermic patients and fertile males. Receiver-operating characteristic curve was used to interpret gene expression data. According to our results, a significant decrease in the expression of CD147 gene and an increase in MMPs genes expression were observed in infertile patients compared to fertile males. These results proved this fact that the CD147 gene has an important role in the regulation of germ cells apoptosis via a MMPs-dependent pathway.
Insights
The CD147 gene is crucial for germ cell survival, regulating apoptosis via matrix metalloproteinases (MMPs). Reduced CD147 and increased MMPs expression in infertile men suggest a MMPs-dependent pathway impacting male fertility.
Area of Science:
- Reproductive Biology
- Molecular Biology
- Cell Biology
Background:
- CD147 is vital for germ cell migration and survival/apoptosis during spermatogenesis.
- The specific role of CD147 in regulating germ cell apoptosis via matrix metalloproteinases (MMPs) remains unelucidated.
Purpose of the Study:
- To investigate the role of CD147 gene expression in regulating germ cell apoptosis.
- To explore the relationship between CD147 and MMPs in male infertility.
Main Methods:
- Real-Time PCR was used to quantify gene expression of CD147, MMP2, MMP7, and MMP9.
- Gene expression data were analyzed using Receiver-Operating Characteristic (ROC) curve analysis.
Main Results:
- A significant downregulation of CD147 gene expression was observed in azoospermic patients compared to fertile males.
- Conversely, a significant upregulation of MMP2, MMP7, and MMP9 gene expression was noted in infertile individuals.
- These findings indicate altered expression patterns of CD147 and MMPs in male infertility.
Conclusions:
- CD147 plays a significant role in the regulation of germ cell apoptosis.
- The apoptosis-regulating function of CD147 is mediated through a matrix metalloproteinase (MMP)-dependent pathway.
- Dysregulation of the CD147-MMP pathway may contribute to male infertility.