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Updated: Jan 31, 2026

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
Published on: October 5, 2012
A DNA minimachine for selective and sensitive detection of DNA
Tatiana A Lyalina1, Ekaterina A Goncharova1, Nadezhda Y Prokofeva1
1ITMO University, Laboratory of Solution Chemistry of Advanced Materials and Technologies, Lomonosova St. 9, 191002, St. Petersburg, Russian Federation. Dmitry.Kolpashchikov@ucf.edu.
Abstract:
Synthetic molecular machines have been explored to manipulate matter at the molecular level. Here, we designed a multifunctional DNA nano-construct, dubbed a 'DNA minimachine' (DMM), which (i) tightly binds complementary DNA; (ii) recognizes specific fragments with high selectivity and (iii) amplifies output signals. DMM1 detects lower concentrations of both single-stranded DNA and double-stranded DNA compared to a conventional probe. This study sets a direction towards the development of molecular machines for selective, sensitive and cost-efficient DNA analysis.
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