Related Experiment Video
Updated: Jan 31, 2026

Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM
Published on: June 27, 2008
Isolation of High-Molecular-Weight DNA from Mouse Yolk Sacs and the Like
Abstract:
Often, genotyping of mouse embryos is required, and a small part, such as the yolk sac, can be used for this purpose. Here, DNA samples are prepared from extra-embryonic tissues by digestion with Proteinase K and subsequent extraction. The yolk sac of mid-gestation or later-stage embryos provides a sufficient amount of DNA for Southern analysis. Small tissues of a few hundred cells are used for genotyping early postimplantation- and preimplantation-stage embryos by polymerase chain reaction (PCR).
Related Concept Videos
Polymers: Defining Molecular Weight
The number average molecular weight (Mn) is the summation of the number...
Polymers: Molecular Weight Distribution
Molecular Weight of Step-Growth Polymers
As the step-growth polymerization involves step-wise condensation of monomers, the molecular weight also builds up eventually. Consequently, high molecular weight polymers are obtained at the late stages of the polymerization, where 99% of monomers have been consumed.
The extent of the...
Anticoagulant Drugs: Low-Molecular-Weight Heparins
DNA Isolation
DNA Isolation

