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Related Concept Videos

DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

113.4K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
113.4K
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

7.4K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
7.4K
Electrophoresis: Overview01:20

Electrophoresis: Overview

4.0K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
4.0K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.2K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
1.2K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.3K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.3K
Silica Gel Column Chromatography: Overview01:10

Silica Gel Column Chromatography: Overview

3.6K
Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
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Related Experiment Video

Updated: Jan 31, 2026

Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

790.1K

Agarose Gel Electrophoresis.

Michael R Green, Joseph Sambrook

    Cold Spring Harbor Protocols
    |January 4, 2019
    PubMed
    Summary

    This protocol details preparing and running agarose gels for DNA visualization. It covers staining DNA using ethidium bromide, SYBR Gold, and SYBR Green 1 for clear results.

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Genetics

    Background:

    • Agarose gel electrophoresis is a fundamental technique for separating DNA fragments.
    • Accurate visualization of DNA is crucial for downstream molecular biology applications.

    Purpose of the Study:

    • To provide a standardized protocol for agarose gel electrophoresis.
    • To compare three common DNA staining dyes: ethidium bromide, SYBR Gold, and SYBR Green 1.

    Main Methods:

    • Detailed steps for preparing agarose gel solutions.
    • Protocols for loading DNA samples and running electrophoresis.
    • Methods for staining gels and visualizing DNA bands.

    Main Results:

    • Successful DNA separation and visualization were achieved using all three dyes.

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    Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins
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    Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins

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    Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis
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    Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis

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    Related Experiment Videos

    Last Updated: Jan 31, 2026

    Agarose Gel Electrophoresis for the Separation of DNA Fragments
    07:10

    Agarose Gel Electrophoresis for the Separation of DNA Fragments

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    Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins
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    Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis
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  • Comparative analysis of staining intensity and safety profiles of ethidium bromide, SYBR Gold, and SYBR Green 1.

    • Conclusions:

    • The protocol provides a reliable method for DNA gel electrophoresis and staining.
    • SYBR Gold and SYBR Green 1 offer potentially safer alternatives to ethidium bromide for DNA visualization.