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MicroRNA expression profiling in human acute organophosphorus poisoning and functional analysis of dysregulated

Haijun Yuan1, Mei Yuan2, Yonghong Tang2

  • 1The Second Affiliated Hospital, University Of South China, Department of Emergency.

African Health Sciences
|January 4, 2019
PubMed
Abstract

Insights

This study identified 37 microRNAs (miRNAs) in the blood of organophosphorus (OP) pesticide poisoning patients. These miRNAs are linked to damage in muscles, nerves, and the heart, offering insights into OP toxicity mechanisms.

Area of Science:

  • Toxicology
  • Molecular Biology
  • Biochemistry

Background:

  • Acute organophosphorus (OP) pesticide poisoning causes multi-organ dysfunction, particularly affecting skeletal muscles, the nervous system, and the heart.
  • The specific microRNA (miRNA) regulatory mechanisms underlying acute OP poisoning-induced organ damage remain largely unexplored.

Purpose of the Study:

  • To investigate the serum miRNA expression profiles in patients with acute OP pesticide poisoning.
  • To elucidate the molecular mechanisms and pathways involved in OP toxicity through miRNA target analysis.

Main Methods:

  • Serum samples from OP-poisoned patients and healthy controls were analyzed using TaqMan Human MicroRNA Array.
  • Quantitative PCR was employed for miRNA validation.
  • Bioinformatic tools were used for miRNA target prediction and pathway analysis.

Main Results:

  • A total of 37 miRNAs exhibited significant differential expression in the sera of OP-poisoned patients compared to controls (29 up-regulated, 8 down-regulated).
  • Functional enrichment analysis revealed that predicted targets of these differentially expressed miRNAs are predominantly associated with pathways implicated in skeletal muscle, nervous system, and cardiac disorders.

Conclusions:

  • This study provides a comprehensive map of serum miRNA expression alterations in acute OP poisoning.
  • The findings highlight potential functional roles of specific miRNAs and their targets in the pathogenesis of OP-induced organ injury, serving as a valuable resource for future research.
Keywords:
MicroRNAacute organophosphorus pesticide poisoningexpression profileshumansignaling pathways

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