Related Experiment Video
Updated: Jan 31, 2026
08:31
Three-dimensional Optical-resolution Photoacoustic Microscopy
Published on: May 3, 2011
18.9K
Photoacoustic microscopy: principles and biomedical applications.
1Department of Biomedical Engineering, Duke University, 100 Science Drive, Durham, NC 27708 USA.
Biomedical Engineering Letters
|January 4, 2019
Summary
Photoacoustic microscopy (PAM) offers detailed anatomical, functional, and molecular insights for biomedical uses. This review covers PAM principles, applications, and challenges for clinical translation.
Area of Science:
- Biomedical Optics
- Medical Imaging Technology
- Photoacoustic Imaging
Background:
- Photoacoustic microscopy (PAM) is a rapidly advancing imaging technique.
- It provides multi-modal information including anatomical, functional, and molecular data.
- PAM is increasingly adopted for diverse biomedical research applications.
Purpose of the Study:
- To provide a concise review of Photoacoustic Microscopy (PAM).
- To discuss the fundamental principles, system designs, and imaging characteristics of PAM.
- To highlight the biomedical applications and future clinical challenges of PAM.
Main Methods:
- Review of basic principles and system designs of PAM (optical-resolution PAM and acoustic-resolution PAM).
- Detailed discussion of PAM imaging characteristics: spatial resolution, penetration depth, and scanning methods.
- Exploration of biomedical applications and technical challenges.
Main Results:
- PAM systems offer versatile imaging capabilities.
- Applications span cellular to organismal levels, including label-free functional and molecular imaging.
- Key imaging characteristics like resolution and depth are detailed.
Conclusions:
- PAM is a powerful tool for biomedical imaging, offering anatomical, functional, and molecular information.
- Significant progress has been made in its applications, from cellular to organismal scales.
- Addressing technical and engineering challenges is crucial for its clinical translation.
Related Concept Videos
01:17Bernoulli's Principle: Applications
6.7K
There are many devices and situations in which fluid flows at a constant height and so can be analyzed using Bernoulli's principle. These devices include, but are not limited to, entrainment devices and fluid flow measuring devices.
Entrainment devices use a high fluid speed to create low pressures and, thus, entrain one fluid into another. Some examples of these devices are given below:
Entrainment devices use a high fluid speed to create low pressures and, thus, entrain one fluid into another. Some examples of these devices are given below:
6.7K
01:11Design Example: Application of Archimedes' Principle
838
Archimedes' principle is fundamental in analyzing the buoyant force and stability of floating bodies. In this example, a wooden block with a rectangular section floats in seawater. Based on the block's dimensions, its specific gravity and the specific weight of seawater are used to find the volume of water displaced and the center of buoyancy.
The volume of seawater displaced by the block is determined by first calculating the block's weight. This is done by multiplying the...
The volume of seawater displaced by the block is determined by first calculating the block's weight. This is done by multiplying the...
838
04:08The Uncertainty Principle
31.8K
Werner Heisenberg considered the limits of how accurately one can measure properties of an electron or other microscopic particles. He determined that there is a fundamental limit to how accurately one can measure both a particle’s position and its momentum simultaneously. The more accurate the measurement of the momentum of a particle is known, the less accurate the position at that time is known and vice versa. This is what is now called the Heisenberg uncertainty principle. He...
31.8K
01:49Hardy-Weinberg Principle
76.3K
Diploid organisms have two alleles of each gene, one from each parent, in their somatic cells. Therefore, each individual contributes two alleles to the gene pool of the population. The gene pool of a population is the sum of every allele of all genes within that population and has some degree of variation. Genetic variation is typically expressed as a relative frequency, which is the percentage of the total population that has a given allele, genotype or phenotype.
76.3K
03:06The Pauli Exclusion Principle
59.3K
The arrangement of electrons in the orbitals of an atom is called its electron configuration. We describe an electron configuration with a symbol that contains three pieces of information:
59.3K
03:02The Aufbau Principle and Hund's Rule
72.7K
To determine the electron configuration for any particular atom, we can build the structures in the order of atomic numbers. Beginning with hydrogen, and continuing across the periods of the periodic table, we add one proton at a time to the nucleus and one electron to the proper subshell until we have described the electron configurations of all the elements. This procedure is called the aufbau principle, from the German word aufbau (“to build up”). Each added electron occupies the...
72.7K