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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Induced Pluripotent Stem Cells01:06

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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
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Embryonic Stem Cells00:58

Embryonic Stem Cells

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Adult Stem Cells01:33

Adult Stem Cells

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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Combinatorial Gene Control02:33

Combinatorial Gene Control

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
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Related Experiment Video

Updated: Jan 31, 2026

Generation of Induced Pluripotent Stem Cells from Muscular Dystrophy Patients: Efficient Integration-free Reprogramming of Urine Derived Cells
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Highly efficient reprogramming and characterization of induced pluripotent stem cells by using a microwell array.

Hyun Lee1,2, Gyu Man Kim3, Jin Ho Choi3

  • 11Stem Cell Neuroplasticity Research Group, Kyungpook National University, Daegu, Korea.

Tissue Engineering and Regenerative Medicine
|January 4, 2019
PubMed
Summary

Researchers developed a novel polydimethylsiloxane stencil system to significantly improve the efficiency of generating induced pluripotent stem cells (iPSCs) from human fibroblasts. This cost-effective method enhances iPSC generation and characterization for patient-specific cell applications.

Keywords:
Induced pluripotent stem cellsPoly(ethylene terephthalate)PolydimethylsiloxaneScissile microarrayStencil

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Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
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RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
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Generation of Induced Pluripotent Stem Cells from Muscular Dystrophy Patients: Efficient Integration-free Reprogramming of Urine Derived Cells
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Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
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RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
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RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Published on: November 26, 2018

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Area of Science:

  • Stem Cell Biology
  • Biotechnology
  • Regenerative Medicine

Background:

  • Induced pluripotent stem cells (iPSCs) offer potential for patient-specific cell therapies.
  • Low efficiency in iPSC generation currently limits their clinical and research applications.
  • Developing efficient reprogramming methods is crucial for advancing stem cell research.

Purpose of the Study:

  • To develop a high-efficiency system for generating induced pluripotent stem cells (iPSCs) from human fibroblasts.
  • To create a method for efficient localization and reprogramming of fibroblasts using a polydimethylsiloxane stencil.
  • To establish a multiple analysis system for characterizing iPSCs derived from the same sample.

Main Methods:

  • Utilized a polydimethylsiloxane stencil for localized reprogramming of human fibroblasts.
  • Developed a micro-patterned scissile microarray for iPSC characterization.
  • Cultured iPSCs on a scissile poly(ethylene terephthalate) sheet for subsequent multi-analysis.

Main Results:

  • Achieved a 2-4 fold increase in iPSC generation efficiency compared to conventional methods.
  • Demonstrated precise control over iPSC culture in micro-patterns.
  • Enabled multiple analyses of iPSC pluripotency from a single sample.

Conclusions:

  • The developed stencil-based system offers a simple, cost-effective, and highly efficient approach for iPSC generation.
  • This method significantly improves the efficiency of reprogramming fibroblasts into iPSCs.
  • The system facilitates robust characterization of iPSCs, supporting the development of patient- and disease-specific pluripotent stem cells.