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Evaluation of Platelet-Rich Plasma and Neutrophil Antimicrobial Extract as Two Autologous Blood-Derived Agents

Tomasz Szponder1, Joanna Wessely-Szponder2, Anna Smolira3

  • 11Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences, Głęboka 30, 20-612 Lublin, Poland.

Tissue Engineering and Regenerative Medicine
|January 4, 2019
PubMed

Insights

Platelet-rich plasma (PRP) and antimicrobial peptide (AMP) extracts from rabbit neutrophils were analyzed. AMP extract suppressed inflammatory responses in macrophages and neutrophils, indicating potential therapeutic applications in tissue healing.

Area of Science:

  • Biomedical Sciences
  • Immunology
  • Regenerative Medicine

Background:

  • Autologous blood-derived preparations like platelet-rich plasma (PRP) and neutrophil extracts offer potential for local tissue healing.
  • Antimicrobial peptides (AMPs) are crucial components of the innate immune system with diverse biological activities.
  • Understanding the in vitro effects of these preparations on immune cells is vital for assessing their therapeutic efficacy and safety.

Purpose of the Study:

  • To characterize platelet-rich plasma (PRP) and antimicrobial peptide (AMP) extract from rabbit neutrophils.
  • To investigate the in vitro effects of AMP extract on macrophage and neutrophil functions, specifically inflammatory responses.
  • To evaluate the potential of these autologous preparations in modulating immune cell activity for enhanced tissue healing.

Main Methods:

  • Preparation of autologous platelet-rich plasma (PRP) and neutrophil-derived antimicrobial peptide (AMP) extract from rabbit blood.
  • Qualitative analysis of PRP and AMP extract using MALDI TOF mass spectrometry to identify key components.
  • In vitro assessment of AMP extract's influence on macrophage superoxide anion generation and neutrophil degranulation and respiratory burst.

Main Results:

  • PRP was found to contain growth factors like PDGF and VEGF, and microbicidal proteins.
  • AMP extract contained various alpha-defensins (NP-1 to -5) and cathelicidins, including the 15-kDa antibacterial protein (p15s).
  • AMP extract significantly decreased superoxide anion generation in macrophages and inhibited degranulation and respiratory burst in neutrophils, suppressing their pro-inflammatory effects.

Conclusions:

  • Autologous PRP and AMP extracts are rich in bioactive molecules with potential therapeutic applications.
  • The AMP extract exhibits immunomodulatory properties by suppressing inflammatory responses in macrophages and neutrophils.
  • These findings suggest that AMP extract, derived from neutrophils, could be a valuable component in strategies aimed at promoting tissue healing by modulating inflammation.
Keywords:
Antimicrobial peptides neutrophil extractCathelicidinPlatelet-rich plasma

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