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Related Concept Videos

Embryonic Stem Cells00:58

Embryonic Stem Cells

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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Culture and Maintenance of Human Embryonic Stem Cells
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Clean-Up Human Embryonic Stem Cell Lines Using Humanized Culture Condition.

Jin Ah Baek1, Hye Won Seol1, Juwon Jung1

  • 11Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, 71, Ihwajang-gil, Jongno-gu, Seoul, 03087 Korea.

Tissue Engineering and Regenerative Medicine
|January 4, 2019
PubMed
Summary
This summary is machine-generated.

Human embryonic stem cells (hESCs) were adapted to xeno-free conditions to eliminate xenogeneic contamination indicators like N-glycolylneuraminic acid (Neu5Gc). This method effectively produced clinical-grade hESCs without compromising pluripotency.

Keywords:
Human embryonic stem cellsHumanized culture conditionNeu5GcXeno-free

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Area of Science:

  • Stem Cell Biology
  • Biotechnology
  • Immunology

Background:

  • Human embryonic stem cell (hESC) culture is transitioning to xeno-free conditions for therapeutic applications.
  • N-glycolylneuraminic acid (Neu5Gc) is a key indicator of xenogeneic contamination, as humans cannot produce it.
  • Established hESC lines require adaptation to meet clinical-grade standards.

Purpose of the Study:

  • To establish a humanized culture condition for hESCs.
  • To evaluate the effectiveness of sequential and direct adaptation methods.
  • To confirm the removal of Neu5Gc and maintenance of pluripotency in adapted hESCs.

Main Methods:

  • Humanized culture media and commercially available humanized materials were utilized.
  • Two adaptation methods (sequential and direct) were employed for hESC lines (SNUhES4 and H1).
  • hESCs were examined for Neu5Gc presence and pluripotency markers post-adaptation.

Main Results:

  • hESCs maintained their characteristic morphology and pluripotency after adaptation.
  • Neu5Gc contamination was undetectable within two passages in the humanized culture system.
  • Both sequential and direct adaptation methods proved effective.

Conclusions:

  • The developed humanized culture system effectively removes xenogeneic Neu5Gc contamination from established hESC lines.
  • This method facilitates the production of clinical-grade hESCs.
  • The adaptation process preserves hESC pluripotency, essential for therapeutic use.