Related Experiment Video
Updated: Jan 31, 2026

Treating Surfaces with a Cold Atmospheric Pressure Plasma using the COST-Jet
Published on: November 2, 2020
Synergistic Effect of Cold Plasma Treatment and RGD Peptide Coating on Cell Proliferation over Titanium Surfaces
Ozan Karaman1, Seyfi Kelebek2, Emine Afra Demirci1
11Tissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical Engineering, Faculty of Engineering and Architecture, Rm 148, İzmir Katip Çelebi University, 35620 İzmir, Turkey.
Abstract:
The aim of this study was to investigate the synergistic effect of cold atmospheric plasma (CAP) treatment and RGD peptide coating for enhancing cellular attachment and proliferation over titanium (Ti) surfaces. The surface structure of CAP-treated and RGD peptide-coated Ti discs were characterized by contact angle goniometer and atomic force microscopy. The effect of such surface modification on human bone marrow derived mesenchymal stem cells (hMSCs) adhesion and proliferation was assessed by cell proliferation and DNA content assays. Besides, hMSCs' adhesion and morphology on surface modified Ti discs were observed via fluorescent and scanning electron microscopy. RGD peptide coating following CAP treatment significantly enhanced cellular adhesion and proliferation among untreated, CAP-treated and RGD peptide-coated Ti discs. The treatment of Ti surfaces with CAP may contribute to improved RGD peptide coating, which enables increased cellular integrations with the Ti surfaces.
Related Concept Videos
Responses to Heat and Cold Stress
Cells Coordinate Growth and Proliferation
Peptide Bonds
Cell-surface Signaling
Abnormal Proliferation
Cold Weather Concreting
To counteract the negative impacts of cold weather, ensuring...

