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Updated: Jan 31, 2026

Relating Stomatal Conductance to Leaf Functional Traits
Published on: October 12, 2015
Formulation and chemical characterization of Clerodendrum infortunatum leaf extract in relation to anti-fungal
Soumen Saha1, Ayan Mukherjee2, Subrata Biswas1
1Department of Chemistry, University of Kalyani, Nadia, Kalyani, 741235, West Bengal.
Abstract:
The study explored to develop an eco-friendly herbal fungicide from chloroform extract of Clerodendrum infortunatum leaves. The extract upon activity guided purification using flash chromatography yielded eight fractions F3 through F10. Total phenol and flavonoid contents in the fractions ranged from 0.12-48.25 mg GAE/g and 0.03-25.29 mg QE/g. LC-MS/MS analysis confirmed the identification of seven phenolic acids across different fractions, the total of which varied between 0 and 2.17 mg/g. Emulsifiable Concentrate (20%) formulation was made with the extract and fractions and tested against Phomopsis vexans causing fruit rot disease in brinjal. Of the various fractions, F8 displayed highest antifungal activity (ED50 = 46.8 μg/ml). Antifungal activity of leaf extract/fractions was correlated with total phenol, total flavonoid and total phenolic acids (r = -0.60 to -0.69). Among the phenolic acids, benzoic acid showed maximum antifungal activity followed by t-cinnamic acid. The relationship between phenolic composition and activity is also reported.
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