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Immobilization pattern of morphologically different microorganisms on bacterial cellulose membranes
Anna Żywicka1, Karolina Wenelska2, Adam Junka3
1Department of Immunology, Microbiology and Physiological Chemistry, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, Piastów 45, 70-311, Szczecin, Poland.
World Journal of Microbiology & Biotechnology
|January 4, 2019
Summary
Microbial cell immobilization on bacterial cellulose (BC) depends on cell shape and BC properties. Optimal carrier selection is crucial for efficient adsorption, multiplication, and controlled release of immobilized microorganisms.
Area of Science:
- Biotechnology
- Materials Science
- Microbiology
Background:
- Bacterial cellulose (BC) is a promising biomaterial for cell immobilization.
- Understanding immobilization patterns is key for optimizing biocatalytic processes.
Purpose of the Study:
- To evaluate the immobilization of diverse microorganisms (Lactobacillus delbruecki, Saccharomyces cerevisiae, Yarrowia lipolytica) on bacterial cellulose.
- To analyze the influence of cell morphology and BC properties on adsorption, incubation, and release efficiencies.
Main Methods:
- The 'adsorption-incubation' method was employed for microbial cell immobilization.
- Assessed adsorption efficiency, cell multiplication (incubation efficiency), and cell release from the BC carrier.
Main Results:
- Immobilization efficiency correlated with BC surface area and cell morphology; smaller cells achieved higher loading.
- Immobilized cell numbers significantly increased during incubation.
- Yarrowia lipolytica's hyphae form led to deep penetration and reduced release, unlike Lactobacillus delbruecki and Saccharomyces cerevisiae.
Conclusions:
- Bacterial cellulose carrier selection must be tailored to specific microbial cell types, considering porosity.
- Cell morphology significantly impacts immobilization efficiency and release characteristics.
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