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Chromatographic Purification of Highly Active Yeast Ribosomes
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Determination of localization of ribonuclease in Aspergillus clavatus

E V Letunova1, L A Ermekbaeva, L N Kozlovskaya

  • 1Bach Institute of Biochemistry, USSR Academy of Sciences, Moscow.

Folia Microbiologica
|January 1, 1988
PubMed

Abstract:

Indirect fluorescent antibody method was used to demonstrate the localization of A. clavatus ribonuclease in apical cell ends. Using protoplasts, ribonuclease was found to be present mainly in the periplasmic space.

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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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A comparative study on the catalytic properties of guanyl-specific ribonucleases.

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[Ribonuclease Ap1 from Aspergillus pallidus. Purification, primary structure and crystallization].

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[Amino acid sequence of ribonuclease Ap1 from Aspergillus pallidus].

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