Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of Complex Ions03:45

Formation of Complex Ions

26.1K
A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
26.1K
DC Generator01:19

DC Generator

2.1K
An alternator converts mechanical energy into electrical energy that varies sinusoidally, resulting in AC current. Meanwhile, a DC generator converts mechanical energy into electrical energy, which are DC pulses with the same polarity. The construction of a DC generator is similar to that of an alternator, except that the pair of slip rings is replaced by a single split ring, also called a commutator. The commutator functions like a periodic rotary switch; it changes the contacts with the...
2.1K
Switching of BJT01:22

Switching of BJT

857
Switching behavior in Bipolar Junction Transistors (BJTs) is a fundamental aspect utilized in various electronic circuits, particularly for digital logic applications like switches and amplifiers. In a typical switching circuit, a BJT alternates between cut-off and saturation modes, corresponding to the "off" and "on" states, respectively, thus behaving like an ideal switch.
Cut-off Mode ("Off" State): In this state, both the emitter-base and collector-base junctions are...
857
DC Battery01:21

DC Battery

1.3K
A conductor needs to be a component of a path that creates a closed loop or full circuit to have a continuous current flowing through it. A current starts to flow if an electric field is created inside an isolated conductor that is not part of a full circuit. The conductor quickly develops a net positive charge at one end and a net negative charge at the other. These charges generate an electric field opposite the direction of the applied electric field, which reduces the current. Eventually,...
1.3K
GTPases and their Regulation02:14

GTPases and their Regulation

9.8K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
9.8K
Bacterial Signaling01:30

Bacterial Signaling

40.6K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
40.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aridity-induced energy reallocation from green to brown food webs predicts grassland carbon storage.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Maize growth as a function of cover crop-mediated soil microbiome.

The New phytologist·2025
Same author

An integrated fast-slow plant and nematode economics spectrum predicts soil organic carbon dynamics during natural restoration.

The New phytologist·2024
Same author

Evolutionarily conserved core microbiota as an extended trait in nitrogen acquisition strategy of herbaceous species.

The New phytologist·2024
Same author

Nutrient-induced acidification modulates soil biodiversity-function relationships.

Nature communications·2024
Same author

Management effects on soil nematode abundance differ among functional groups and land-use types at a global scale.

The Journal of animal ecology·2022

Related Experiment Video

Updated: Jan 31, 2026

High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence
07:25

High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence

Published on: February 23, 2021

4.5K

A switch in the poly(dC)/RmlB complex regulates bacterial persister formation.

Xu Chen1, Gen Li1, Xuewei Liao2

  • 1Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.

Nature Communications
|January 4, 2019
PubMed
Summary
This summary is machine-generated.

Bacterial persister cells, crucial for chronic infections, are linked to a novel poly(dC)/RmlB complex in Pseudomonas aeruginosa. Environmental triggers alter this complex, inducing dormancy by reducing cellular energy and impacting antibiotic tolerance.

More Related Videos

Real-Time DC-dynamic Biasing Method for Switching Time Improvement in Severely Underdamped Fringing-field Electrostatic MEMS Actuators
11:44

Real-Time DC-dynamic Biasing Method for Switching Time Improvement in Severely Underdamped Fringing-field Electrostatic MEMS Actuators

Published on: August 15, 2014

10.8K
Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli
12:29

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli

Published on: March 24, 2023

2.7K

Related Experiment Videos

Last Updated: Jan 31, 2026

High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence
07:25

High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence

Published on: February 23, 2021

4.5K
Real-Time DC-dynamic Biasing Method for Switching Time Improvement in Severely Underdamped Fringing-field Electrostatic MEMS Actuators
11:44

Real-Time DC-dynamic Biasing Method for Switching Time Improvement in Severely Underdamped Fringing-field Electrostatic MEMS Actuators

Published on: August 15, 2014

10.8K
Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli
12:29

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli

Published on: March 24, 2023

2.7K

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Molecular Biology

Background:

  • Bacterial persisters are dormant cells that survive antibiotic treatment, contributing to persistent and recurrent infections.
  • Understanding the molecular mechanisms of persister formation is critical for developing new therapeutic strategies against challenging infections.

Purpose of the Study:

  • To investigate the role of a novel extracellular poly(dC) and membrane-associated RmlB protein complex in Pseudomonas aeruginosa persistence.
  • To elucidate the signaling pathways and cellular changes associated with this complex's role in bacterial dormancy and antibiotic tolerance.

Main Methods:

  • Characterization of the poly(dC)/RmlB complex and its environmental state switching in Pseudomonas aeruginosa.
  • Analysis of cellular physiological changes, including proton motive force and ATP levels, upon complex state alteration.
  • Investigation of the involvement of (p)ppGpp signaling, inorganic polyphosphate, Lon protease, exonuclease VII, and type III secretion system.

Main Results:

  • A complex of extracellular poly(dC) and membrane-associated RmlB is identified and linked to Pseudomonas aeruginosa persistence.
  • Environmental stimuli induce a switch in the poly(dC)/RmlB complex, leading to decreased proton motive force and ATP levels, promoting cell dormancy.
  • This process is regulated by a (p)ppGpp-controlled pathway involving key cellular components and potentially serves as an adaptive response to dTDP-L-rhamnose pathway disruption.

Conclusions:

  • The poly(dC)/RmlB complex represents a novel mechanism contributing to bacterial persistence in Pseudomonas aeruginosa.
  • Modulation of this complex and its associated signaling pathway offers potential targets for combating chronic and antibiotic-resistant infections.