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Photoactive chelates for radiolabelling proteins.

Larissa S Eichenberger1, Malay Patra, Jason P Holland

  • 1University of Zurich, Department of Chemistry, Winterthurerstrasse 190, CH-8057, Zurich, Switzerland. jason.holland@chem.uzh.ch.

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Photochemical reactions enable rapid, single-step synthesis of radiolabeled proteins like antibodies. This study details new chelates for 68Ga radiochemistry, proving effective for protein labeling via photochemistry.

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Area of Science:

  • Bioconjugation Chemistry
  • Radiopharmaceutical Chemistry
  • Photochemistry

Background:

  • Photochemical reactions offer a promising route for synthesizing radiolabeled biomolecules.
  • Developing efficient methods for radiolabeling proteins, such as antibodies and peptides, is crucial for diagnostics and therapeutics.
  • Macrocyclic chelates are essential for stable radiometal complexation.

Purpose of the Study:

  • To synthesize and characterize novel macrocyclic chelates functionalized with an arylazide group for radiolabeling applications.
  • To investigate the 68Ga-radiochemistry and photochemical reactivity of these new chelates.
  • To demonstrate the utility of photoradiochemistry for the rapid, one-pot synthesis of radiolabeled proteins using an antibody as a model.

Main Methods:

  • Synthesis of three macrocyclic chelates incorporating an arylazide moiety.
  • Evaluation of 68Ga complexation and radiolabeling efficiency.
  • Photochemical activation and conjugation of chelates to proteins (trastuzumab).
  • Analysis of radiolabeled protein purity and stability.

Main Results:

  • Successful synthesis of three functionalized macrocyclic chelates.
  • Efficient complexation with Gallium-68 (68Ga) and high radiochemical yields achieved.
  • Photochemical conjugation to trastuzumab demonstrated fast, one-pot radiolabeling under mild conditions.
  • High purity of the final radiolabeled antibody confirmed.

Conclusions:

  • The developed macrocyclic chelates are suitable for 68Ga radiochemistry and efficient protein photolabeling.
  • Photoradiochemistry provides a rapid and convenient method for synthesizing radiolabeled proteins.
  • This approach holds potential for the streamlined production of diagnostic and therapeutic radiopharmaceuticals.