Flexible Nanopipettes for Minimally Invasive Intracellular Electrophysiology In Vivo
Krishna Jayant1, Michael Wenzel2, Yuki Bando2
1Department of Electrical Engineering, Columbia University, New York, NY 10027, USA; Department of Biological Sciences, Columbia University, New York, NY 10027, USA; NeuroTechnology Center, Columbia University, New York, NY 10027, USA; Kavli Institute for Brain Science, Columbia University, New York, NY 10027, USA.
Cell Reports
|January 4, 2019
Summary
Flexible quartz nanopipettes offer stable in vivo intracellular recordings, improving accuracy and duration. This breakthrough technique links neuron electrical properties to network function, even during seizures.
Area of Science:
- Neuroscience
- Biophysics
- Materials Science
Background:
- Intracellular recordings in vivo are crucial for understanding neural network function.
- Current methods face limitations in accuracy, throughput, duration, and stability due to factors like washout and membrane damage.
Purpose of the Study:
- To introduce flexible quartz nanopipettes as an improved tool for in vivo intracellular recordings.
- To overcome the limitations of traditional microelectrodes for stable and precise neural recordings.
Main Methods:
- Development and application of flexible quartz nanopipettes (10-25 nm inner diameter).
- Utilizing nanopipettes for stable intracellular recordings in anesthetized and awake mice (seal resistances 500-800 MΩ, 5-10 cells/nanopipette, ~1 hr duration).
- Combining nanopipettes with quantum-dot labels and microprisms for two-photon targeted electrophysiology and simultaneous population imaging.
Main Results:
- Achieved stable intracellular recordings with high seal resistances and multi-cell recording capability.
- Demonstrated minimal diffusional flux and precise recording/stimulation capabilities.
- Successfully recorded from parvalbumin-positive interneurons during seizure propagation, observing depolarization block coinciding with epileptic spread.
Conclusions:
- Flexible quartz nanopipettes provide a simple and effective method for stable in vivo intracellular recordings.
- This technique enhances the ability to link single-neuron activity to network dynamics and investigate neurological conditions like epilepsy.
- The method enables advanced applications such as targeted electrophysiology and simultaneous imaging across cortical layers.
Keywords:
dendritesimaginginter-neuronintracellularin vivonanoelectrodenanopipetteseizuretargetedtwo-photonMore Related Videos
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