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Updated: Jan 31, 2026

A Neural Network-Based Identification of Developmentally Competent or Incompetent Mouse Fully-Grown Oocytes
Published on: March 3, 2018
Di(n-butyl) phthalate exposure impairs meiotic competence and development of mouse oocyte
Fei-Ping Li1, Jie-Long Zhou2, Ai-Wei Guo2
1Organ Transplantation Institute, Medical College, Xiamen University, Xiamen, Fujian, China; College of Life Sciences, Southwest Forestry University, Kunming, Yunnan, China; Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen, Fujian, China; Chengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, Sichuan, China.
Abstract:
Di(n-butyl) phthalate (DBP) is extensively used in industrial applications as plasticizer and stabilizer and its presence in the environment may present health risks for human. Previous studies have demonstrated its mutagenic, teratogenic, and carcinogenic ability. However, its effect on mammalian oocyte maturation remains unknown. In this study, we examined the effect of DBP on oocyte maturation both in vitro and in vivo. Our results showed that DBP could significantly reduce mice oocyte germinal vesicle breakdown (GVBD) and polar body extrusion (PBE) rates. In addition, oocyte cytoskeleton was damaged and cortical granule-free domains (CGFDs) were also disrupted. Finally, DBP induced early apoptosis of oocyte and granulosa cells (GCs). Collectively, these data demonstrate that DBP could reduce meiosis competence and mouse oocyte development.
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