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Updated: Jan 31, 2026

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells
Published on: February 12, 2021
Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults
Katerina Vlahos1, Koula Sourris1, Robyn Mayberry1
1Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, Victoria 3052, Australia.
Abstract:
We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms.
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