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Updated: Jan 31, 2026

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
Published on: October 2, 2017
Andrographolide is neither a human organic anion transporter 1 (hOAT1) substrate nor inhibitor
Yoong Min Chong1, Gurjeet Kaur2, Mei Lan Tan1,3
1a Malaysian Institute of Pharmaceuticals and Nutraceuticals, NIBM , Ministry of Science Technology and Innovation (MOSTI) , Pulau Pinang , Malaysia.
Abstract:
Andrographolide, a major bioactive compound isolated from Andrographis paniculata (Burm. F.) Nees, was evaluated for its effects on the hOAT1 membrane transporter. Substrate determination and inhibition of hOAT1-mediated uptake transport assay was carried out using recombinant CHO-hOAT1 cells. The results showed that the uptake ratio of andrographolide was less than 2.0 at all concentrations tested, indicating that andrographolide is not a hOAT1 substrate. Andrographolide has no significant effects on the p-aminohippuric acid uptake and on the mRNA and protein expression of hOAT1. In conclusion, andrographolide may not pose a drug-herb interaction risk related to hOAT1.
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