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Updated: Jan 31, 2026

Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
Published on: April 17, 2009
Dual RNA-seq analysis provides new insights into interactions between Norway spruce and necrotrophic pathogen
Andriy Kovalchuk1, Zhen Zeng2, Rajendra P Ghimire3
1Department of Forest Sciences, Faculty of Agriculture and Forestry, University of Helsinki, P.O. Box 27, FIN-00014, Helsinki, Finland. andriy.kovalchuk@helsinki.fi.
Background:
Root and butt rot of conifer trees caused by fungi belonging to the Heterobasidion annosum species complex is one of the most economically important fungal diseases in commercial conifer plantations throughout the Northern hemisphere. We investigated the interactions between Heterobasidion fungi and their host by conducting dual RNA-seq and chemical analysis on Norway spruce trees naturally infected by Heterobasidion spp. We analyzed host and pathogen transcriptome and phenolic and terpenoid contents of the spruce trees.
Results:
Presented results emphasize the role of the phenylpropanoid and flavonoid pathways in the chemical defense of Norway spruce trees. Accumulation of lignans was observed in trees displaying symptoms of wood decay. A number of candidate genes with a predicted role in the higher level regulation of spruce defense responses were identified. Our data indicate a possible role of abscisic acid (ABA) signaling in the spruce defense against Heterobasidion infection. Fungal transcripts corresponding to genes encoding carbohydrate- and lignin-degrading enzymes, secondary metabolism genes and effector-like genes were expressed during the host colonization.
Conclusions:
Our results provide additional insight into defense strategies employed by Norway spruce trees against Heterobasidion infection. The potential applications of the identified candidate genes as markers for higher resistance against root and butt rot deserve further evaluation.
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