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Routine Screening Method for Microparticles in Platelet Transfusions
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Caspase 3/ROCK1 pathway mediates high glucose-induced platelet microparticles shedding

Gui Hua Wang1, Kun Ling Ma1, Yang Zhang1

  • 1Institute of Nephrology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009, China.

Biochemical and Biophysical Research Communications
|January 5, 2019
PubMed
Abstract

Insights

High glucose increases platelet microparticles (PMPs) shedding in diabetes. This occurs through the caspase3-ROCK1 signaling pathway, offering potential therapeutic targets for diabetic complications.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Diabetology

Background:

  • Platelet microparticles (PMPs) are linked to macrovascular issues in diabetes.
  • Understanding high glucose's role in PMP generation is crucial.

Purpose of the Study:

  • To investigate the mechanisms behind high glucose-induced PMP generation.
  • To identify key molecular players in this process.

Main Methods:

  • Rat platelets were incubated with high glucose.
  • PMP shedding was measured.
  • ROCK1 and caspase3 expression and activity were assessed.

Main Results:

  • High glucose increased PMP shedding, ROCK1, and caspase3 levels.
  • ROCK inhibition blocked PMP shedding.
  • Caspase3 inhibition reduced ROCK1 activity and PMP generation.

Conclusions:

  • High glucose stimulates PMP shedding via the caspase3-ROCK1 pathway.
  • This pathway is a potential target for managing diabetic vascular complications.
Keywords:
High glucosePlatelet microparticlesRho-associated kinase 1caspase3

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