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Updated: Jan 31, 2026

Electrochemiluminescence Assays for Human Islet Autoantibodies
Published on: March 23, 2018
A novel label free electrochemiluminescent aptasensor for the detection of lysozyme
Yasamin Nasiri Khonsari1, Shiguo Sun2
1State Key Laboratory of Fine Chemicals, Dalian University of Technology, No.2, Linggong Road, Ganjingzi District, Dalian 116023, China.
Abstract:
In this paper we describe a novel ultrasensitive electrochemiluminescent (ECL) aptasensor based on the quenching effect of aptamer and lysozyme incubation on co-reactant ECL mechanism of nitrogen-doped graphene quantum dots and persulfate. Incorporation of gold nanoparticles let to enhancement of electron transfer process of co-reactant mechanism species. The electrochemical behavior of each step modification of aptasensor was investigated using electrochemical impedance spectroscopy and verified by ECL responses. The aptasensor showed high stability, sensitivity, reliable reproducibility, wide linear range (10 fM to 10 nM) with a low detection limit of 0.8 fM (at an S/N ratio of 3, n = 10) can be successively applied to the human serum samples analysis. Recoveries ranged between 91 and 104% (with RSDs of <3.1%).
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