Related Experiment Video
Updated: Jan 31, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
Published on: November 4, 2016
Novel one step transformation method for Escherichia coli and Staphylococcus aureus using arginine-glucose
Ketaki Deshmukh1, Sutapa Roy Ramanan2, Meenal Kowshik1
1Biological Sciences Department, Birla Institute of Technology and Science Pilani, K K Birla Goa Campus, Goa, India.
Abstract:
Bacterial gene transformation is one of the important techniques in molecular biology which has significant applications in gene cloning technology. In this study, we have developed arginine-glucose functionalized hydroxyapatite nanoparticle (R-G-HAp NPs) mediated novel one step transformation method, effective for both Gram-positive and Gram-negative bacteria. R-G-HAp NPs served as carriers to deliver pDNA into Escherichia coli and Staphylococcus aureus at room temperature, without the need for preparation of competent cells. High transformation efficiency was achieved in Gram-positive, S. aureus (107 cfu/μg of pDNA) as well as Gram-negative, E. coli (109 cfu/μg of pDNA). This demonstrates the efficacy of R-G-HAp NPs as a nano-vehicle to achieve high plasmid transformation efficiency, even in Gram-positive bacteria which is usually a challenge, exhibiting their potential as promising synthetic non-viral vectors for efficient bacterial gene transformation.
Related Concept Videos
Transformations of Functions I
Transformations of Functions II
Transformations of Functions III
Bacterial Transformation
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Glucose Homeostasis: Regulation of Blood Glucose
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
Energy-requiring Steps of Glycolysis

