Related Experiment Video
Updated: Jan 31, 2026
07:58
Cell-Free DNA Integrity Analysis in Urine Samples
Published on: January 5, 2017
14.3K
Effect of Conventional and Microwave Tissue Processing Technique on DNA Integrity: A Comparative Molecular Analysis
Dhara Dwivedi1, Sowmya Kasetty2, Manisha S Tijare3
1Department of Oral Pathology Dentistry Unit, Ayder Referral Hospital-College of Health Sciences Mekelle University, Ethiopia.
Ethiopian Journal of Health Sciences
|January 5, 2019
Summary
Microwave tissue processing yields DNA comparable in quality and quantity to conventional methods for oral squamous cell carcinoma research. This technique offers a viable alternative for molecular studies.
Area of Science:
- Histopathology
- Molecular Biology
- Biotechnology
Background:
- Conventional tissue processing for molecular diagnostics has limitations.
- Microwave technology is emerging as an alternative in histopathology.
- Evaluating microwave processing for DNA extraction is crucial.
Purpose of the Study:
- To compare DNA yield and quality from conventional vs. microwave tissue processing.
- To assess the suitability of microwave-processed tissues for Polymerase Chain Reaction (PCR).
- To analyze DNA from oral squamous cell carcinoma (OSCC) samples.
Main Methods:
- Thirty OSCC tissue samples were divided into two halves.
- One half underwent conventional manual processing; the other used a domestic microwave.
- DNA was extracted from both, followed by PCR for GAPDH gene expression analysis.
Main Results:
- Microwave processing resulted in a higher DNA yield.
- DNA quality was similar between conventional and microwave methods.
- GAPDH gene expression was successfully evaluated from both sample types.
Conclusions:
- Microwave tissue processing provides DNA of comparable quantity and quality to conventional methods.
- This technique is suitable for molecular studies and research applications.
- Microwave processing is a viable alternative for preparing tissues for molecular analysis.
Related Concept Videos
01:20Comparing the Survival Analysis of Two or More Groups
595
Survival analysis is a cornerstone of medical research, used to evaluate the time until an event of interest occurs, such as death, disease recurrence, or recovery. Unlike standard statistical methods, survival analysis is particularly adept at handling censored data—instances where the event has not occurred for some participants by the end of the study or remains unobserved. To address these unique challenges, specialized techniques like the Kaplan-Meier estimator, log-rank test, and...
595
02:00Molecular Models
43.7K
Physical models representing molecular architectures of chemical compounds play essential roles in understanding chemistry. The use of molecular models makes it easier to visualize the structures and shapes of atoms and molecules.
43.7K
01:30Sign Convention
3.5K
When analyzing a beam subjected to various loads, it is crucial to understand the internal forces and moments generated within the structure. These internal forces can be broadly classified into normal forces, shear forces, and bending moments. To determine these forces and moments, we use the method of sections and apply a specific sign convention based on their direction and the side of the section being analyzed.
The normal force acts perpendicular to the beam's cross-section and can...
The normal force acts perpendicular to the beam's cross-section and can...
3.5K
01:15Enolate Mechanism Conventions
2.9K
When a carbonyl compound is treated with a strong base, the α position gets deprotonated to give a resonance-stabilized intermediate called an enolate. Enolates are ambident nucleophiles because they possess two nucleophilic sites that can attack an electrophile owing to the delocalization of the negative charge between the α carbon and oxygen atoms. When the oxygen atom attacks an electrophile, it is called O-attack, whereas electrophilic attack via the α carbon is known as...
2.9K
02:16Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
16.0K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
16.0K
02:54Molecular and Ionic Solids
20.0K
Crystalline solids are divided into four types: molecular, ionic, metallic, and covalent network based on the type of constituent units and their interparticle interactions.
Molecular Solids
Molecular crystalline solids, such as ice, sucrose (table sugar), and iodine, are solids that are composed of neutral molecules as their constituent units. These molecules are held together by weak intermolecular forces such as London dispersion forces, dipole-dipole interactions, or hydrogen bonds, which...
Molecular Solids
Molecular crystalline solids, such as ice, sucrose (table sugar), and iodine, are solids that are composed of neutral molecules as their constituent units. These molecules are held together by weak intermolecular forces such as London dispersion forces, dipole-dipole interactions, or hydrogen bonds, which...
20.0K